D3.336 - CC variant of the -634G/C polymorphism of the VEGF gene is associated with higher HDL levels in patients with type 1 diabetes mellitus
Background
HDL is a protective factor that reduces the likelihood of cardiovascular events and is also a good absorber of endotoxin, preventing further inflammatory activity. There are many factors affecting HDL concentration and activity. This study assesses whether the -634G/C polymorphism of the VEGF gene affects HDL concentration in patients with type 1 diabetes mellitus.
Method
92 patients with a verified diagnosis of DM1 were assessed. All patients had assessment of the content of HDL (Me (Q1-Q3); mmol/L) in plasma determined by quantitative high-sensitivity enzyme-linked immunosorbent assay using ELISA test manufactured by Cloud Clone corp. (Wuhan, Hubei, China). Allele-specific polymerase chain reaction with electrophoretic detection was used to analyze the VEGF -634G/C polymorphism. DNA was isolated from whole blood of patients with DM1 using the DNA Express blood DNA isolation kit according to the manufacturer's instructions.
Results
HDL level was significantly higher in the group of patients with DM1 and СС variant of -634G/C VEGF polymorphism – 1.58 (1.39-1.89), than in CG variant – 1.21 (0.9-1.58) (p=0.013).
Conclusion
The presence of CC variant -634G/C polymorphism of the VEGF gene is associated with higher HDL values in patients with DM1, which may significantly affect their cardiovascular system and potentially be a protective variant of this gene related to atherosclerosis.
