D2.271 - The effect of Organic Light Emiting Diode(OLED) phototherapy in atopic mouse model

The purpose of this study was to investigate the effects of phototherapy (OLED) in atopic mouse model. Specifically, we aimed to investigate the mechanism and efficacy of phototherapy using an OLED patch in an atopic dermatitis model.

To make atopic dermatitis mice, 10 μl of 5% oxazolone was applied to both ears of wild-type BALB/c mice on Day 0. On Day 5, 25 μl of 0.5% oxazolone was applied to each dorsal area. The mice were treated with 0.5% oxazolone nine times, every other day for a total of 20 days. The dorsal thickness and TEWL of the mice were measured before each 0.5% oxazolone treatment. On Day 20, the dorsal thickness and TEWL were measured, the mice were sacrificed, cardiac blood was collected, and skin biopsies were taken from the oxazolone-sensitized area to measure cytokine expression and histological changes. The TEWL of skin lesions was measured in a group that was exposed to OLED on atopic dermatitis lesions in mice and a control group that was not exposed to OLED. After collecting skin tissue, the expression levels of cytokines associated with atopic dermatitis and inflammation-related factors were measured to analyze the therapeutic effect

In skin tissues induced with atopic dermatitis, the epidermis thickened compared to the normal group. Both the 15-minute and 30-minute OLED groups showed thinner epidermal tissue compared to the normal group. Notably, the 30-minute OLED group showed a similar thinning compared to the 15-minute OLED group. TEWL measurements showed the lowest water evaporation in the 30-minute OLED group. Skin thickness measurements also confirmed that the 30-minute OLED group had the thinnest skin. IgE levels increased in the atopic dermatitis model compared to the normal group. The 15-minute OLED group showed an approximately 10% decrease in IgE levels compared to the atopic dermatitis group. The 30-minute OLED group showed an approximately 70% reduction in IgE levels. Unlike the IgE levels, there was no significant increase in the levels of IL-4 and IL-17 in atopic dermatitis compared to the normal group.

The OLED patch's therapeutic effects were tested in mouse models of atopic dermatitis. The efficacy, safety, and therapeutic effects of the OLED patch were confirmed.