D1.493 - Circulating Exosomal miR-200 Family Downregulation in Allergic Rhinitis

Circulating exosomal microRNAs (miRNAs) reflect systemic immune and epithelial regulatory processes. We aimed to define the exosomal miRNA signature associated with allergic rhinitis (AR).

Plasma-derived exosomes were isolated from patients with AR (n=10) and healthy controls (n=10). Small RNA sequencing was performed followed by differential expression analysis with false discovery rate (FDR) control. miRNAs with FDR<0.05 were considered significant. Receiver operating characteristic (ROC) analysis assessed discriminative performance.

Among 765 detected miRNAs, 38 were significantly differentially expressed (FDR<0.05), with 29 showing |log2 fold change|≥1. Most were downregulated in AR. Notably, multiple miR-200 family members (miR-200c-3p, miR-141-3p, miR-200a-3p) were among the most significantly decreased (log2FC −4.16 to −5.18; all FDR<0.001), indicating a coordinated regulatory signature. Individually, miR-200 family members demonstrated strong discrimination (AUC 0.91–0.95). A combined three-miRNA model achieved an AUC of 0.88 under leave-one-out cross-validation.

AR is associated with decreased circulating exosomal miR-200 family expression, highlighting its potential biomarker relevance.