D3.337 - The -634G/C polymorphism of the VEGF gene affects BPI concentration in patients with type 1 diabetes mellitus
Background
Bactericidal permeability-increasing protein (BPI) is important in host defense against Gram-negative bacteria and endotoxin. Its ability to inhibit neoangiogenesis is a good property especially in patients with DM1. This study assess if the -634G/C polymorphism of the VEGF gene, an important vascular regulator, affects BPI concentration in DM1 patients.
Method
92 patients with a verified diagnosis of DM1 were assessed. All patients had the content of BPI (Me (Q1-Q3); pg/mL) in plasma determined by quantitative high-sensitivity enzyme-linked immunosorbent assay using ELISA test manufactured by Cloud Clone corp. (Wuhan, Hubei, China). Allele-specific polymerase chain reaction with electrophoretic detection was used to analyze the VEGF -634G/C polymorphism. DNA was isolated from whole blood of patients with DM1 using the DNA Express blood DNA isolation kit according to the manufacturer's instructions.
Results
BPI levels weres significantly higher in the group of patients with DM1 and GG variant of -634G/C VEGF polymorphism – 136.5 (57.0-140.0), than in CC variant – 56.0 (54.0-58.5) (p=0.018).
Conclusion
The -634G/C polymorphism of the VEGF gene may affect BPI levels in patients with type 1 DM. A “protective” VEGF genotype may exist, increasing BPI concentration and inhibiting angiogenesis.
