D3.203 - Characterisation of relevant allergens in grass pollen extracts

Grass pollen allergy is a leading cause of seasonal allergic rhinitis worldwide, currently allergen immunotherapy (AIT) predominantly relies on Phleum pratense as a representative species. Recent studies have often focussed on the clinical relevance of group 1 and group 5 grass allergens. However, numerous studies have shown the clinical relevance of other allergen groups, e.g. group 4 and group 13. A broad-spectrum approach to grass pollen AIT is more reflective of natural exposure and holds promise for improved patient outcomes. 

Characterisation of relevant allergens in grass pollen extracts was performed using SDS-PAGE gel slice analysis with LC-MS. Further characterisation of two major grass allergens, group 1 and group 5, was performed using monoclonal Western blots from 13 species of Poaceae family grasses. This was performed using monoclonal antibodies.

Characterisation of relevant allergens within a mixture of 13 grasses using LC-MS led to the identification of 25 allergens. Identification of two major grass allergens group 1 and group 5 was achieved within 13 Poaceae family grasses. The Western blots show differing molecular weights between the species indicating that they have distinguishable properties.

Characterisation of relevant allergens from pollen extracts show that combining pollens into a broad-spectrum product ensures allergomic diversity without dilution of major allergen. A broad-spectrum approach to grass pollen AIT is more reflective of natural exposure and holds promise for improved patient outcomes.