D2.17 - Prostate-Specific Protein Can f 5 as an Allergen in Seminal Fluid Hypersensitivity: A Case Study

Hypersensitivity to seminal fluid is rare but can manifest with significant clinical symptoms. The prostate-specific protein Can f 5, previously implicated in dog allergies, has been identified as a potential allergen in seminal plasma. This study investigates a case of suspected seminal fluid hypersensitivity and its molecular basis.

A 38-year-old female experienced recurrent pharyngeal pain, genital swelling, and systemic symptoms after unprotected intercourse with her partner, lasting for several days. Genital symptoms improved with condom use but recurred upon discontinuation due to attempts to conceive. The patient has no history of reactions with previous partners and reports respiratory allergies to dogs and horses, particularly male dogs.

Protein extracts were prepared from the seminal fluid, saliva, and serum of the patient’s partner using PBS extraction, centrifugation, and filtration. SDS-PAGE and Western blot analyses were performed under reducing and non-reducing conditions. IgE-ELISA and IgG-ELISA were conducted to evaluate specific reactivity to Can f 5 and the seminal fluid proteins

sIgE Can f 5: negative by ImmunoCAP and ALEX-test. However, western blot revealed IgE binding to seminal fluid proteins of approximately 80 kDa and 28 kDa under reducing conditions. The 28 kDa band corresponds to prostate-specific kallikrein (Can f 5). No IgG reactivity was detected in the IgG-Western blot or ELISA. Saliva and serum from the partner did not demonstrate IgE binding, confirming that Can f 5 is restricted to seminal plasma.

This case highlights Can f 5 as a significant allergen in seminal fluid hypersensitivity mediated by IgE. Comprehensive allergenic profiling is crucial for diagnosis and management, particularly for patients with unique allergic triggers. This study underscores the need for tailored interventions to address reproductive challenges in such cases.